The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? Normally, … Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. ⇒ Collect the blood sample from the capillary or venipuncture. This procedure is known as a blood film. The drop should be in the center line of the glass slide. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. 6. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). Save my name, email, and website in this browser for the next time I comment. Blood smears are needed for microscopic examination of the blood. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. The undiluted stain fixes and partially stains the smear. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? A small drop of blood is placed on the midline at the end of a glass slide. Your email address will not be published. Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. ⇒ Allow the blood smear to air-dry completely. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. After staining the slide, the examination begins. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. Preparing the Peripheral Blood Film . My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. There are many reasons why your doctor may order a blood smear. associated with the decrease in the viscosity of blood. Tnx for all keep it up am lab technician from Ethiopia. Maintain an even gentle pressure on the slide. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). It expounds its la … Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. If you continue browsing the site, you agree to the use of cookies on this website. Blood can also be taken from an EDTA sample. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. and the Date on the frosted end of the slide. Keep the same angle all the way to the end of the smear. Wear gloves. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. Thin smears. The most common technique of blood smear preparation is called the “wedge or push” technique. Cover the blood film with undiluted staining solution. This article highlights the basic science and art behind the PBF. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. Preparation of blood smears . Preparation of Blood Smear: Collection of Sample: 1. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube Clean slides with 70 to 90% alcohol and allow to dry. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. blood (within 1 hr. CBC & Peripheral Blood Smear. 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